normal esophageal epithelial cells heec  (Procell Inc)

Journal: Regenerative Therapy

Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

doi: 10.1016/j.reth.2026.101101

Figure Lengend Snippet: MMP12 was identified as a key gene and its expression was upregulated in EC cells. (A) Expression analysis of INHBA, MMP3, MMP12, and SPP1 in EC tissues and normal esophageal tissues through the GEPIA database. (B) Expression analysis of INHBA, MMP3, MMP12, and SPP1 in HEEC and KYSE150 cells. (C) MMP12 expression analysis in pan-cancer tissues and normal tissues through the GEPIA database. (D) qRT-PCR was performed to detect MMP12 protein expression in HEEC and KYSE150 cells. (E) The efficiency of MMP12 knockdown was determined by Western blotting in KYSE150 cells. ns: not significant, ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: EC cell (KYSE-150 and TE-10), normal esophageal epithelial cells (HEEC) and THP-1 cells were provided by Procell (Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Western Blot

Journal: Cancer Medicine

Article Title: The GNL3L ‐ MDM2 Interaction Drives Esophageal Squamous Cell Carcinoma Progression

doi: 10.1002/cam4.71146

Figure Lengend Snippet: Expression of GNL3L in esophageal cancer (EC) and the cell line model of GNL3L knockdown. (a) GNL3L expression was analyzed in EC tumor tissues ( n = 182) and normal esophageal tissues ( n = 286) using the GEPIA database. (b) Kaplan–Meier analysis of survival rates between patients with EC and low expression of GNL3L ( n = 138) vs. high expression of GNL3L ( n = 46). In addition to the expression of GNL3L , the survival rate was also related to tumor grade. The patient cohort was obtained from the gene information of TCGA Esophageal cancer datasets downloaded from the UALCAN website. (c) Immunohistochemistry was performed to detect differential expression of GNL3L protein in an esophageal squamous cell carcinoma (ESCC) specimen. (d) qRT‐PCR was used to detect GNL3L expression in ESCC cell lines (TE‐1, KYSE‐410, KYSE‐30, and EC9706) and human normal esophageal epithelial cell line (HEEC). (e) The expression of GNL3L protein in ESCC cell lines and HEEC was assessed using western blotting (WB). (f) Efficiency of sh‐GNL3L for GNL3L in TE‐1 cells by qRT‐PCR. (g) The protein expression of GNL3L in TE‐1 cells. (NC: Negative control). (* p < 0.05, ** p < 0.01).

Article Snippet: ESCC (TE‐1, KYSE‐410, KYSE‐30, EC9706, and ECA‐109) and human normal esophageal epithelial (HEEC) cell lines were obtained from Procell Life Science &Technology Co. Ltd. (Wuhan, China).

Techniques: Expressing, Knockdown, Immunohistochemistry, Quantitative Proteomics, Quantitative RT-PCR, Western Blot, Negative Control

Journal: Journal of Cancer

Article Title: Identification and Characterization of Oxidative Stress and Endoplasmic Reticulum Stress-Related Genes in Esophageal Cancer

doi: 10.7150/jca.104376

Figure Lengend Snippet: TFRC promotes the proliferation, migration, and invasion of ESCA cells. A TFRC expression levels were analyzed in ESCA cells and HEEC using qRT-PCR. B The transfection efficiency of TFRC overexpression in TE1 cells and knockdown in OE33 cells was confirmed by qRT-PCR. C-D Cell proliferation was evaluated in TFRC-overexpressing TE1 cells and TFRC-knockdown OE33 cells using MTS assays (C) and colony formation assays (D). E-F The migration and invasion capabilities of TFRC-overexpressing TE1 cells and TFRC-knockdown OE33 cells were assessed through transwell migration (E) and invasion (F) assays. Data are presented as the mean ± SD from at least three independent experiments. *P < 0.05.

Article Snippet: Human ESCC cell lines (KYSE150 and TE1), an EAC cell line (OE33), and a human normal esophageal epithelial cell line (HEEC) were obtained from the China Center for Type Culture Collection (CCTCC, Wuhan, China).

Techniques: Migration, Expressing, Quantitative RT-PCR, Transfection, Over Expression, Knockdown